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Sterilization is crucial in the medical field to prevent
infections and ensure patient safety. Various sterilization methods are used
depending on the type of instruments, materials, and level of sterilization
required. Here are the main sterilization processes:
1. Heat-Based Sterilization
- Autoclaving
(Steam Sterilization): Uses high-pressure saturated steam (121–134°C)
for a specific time to kill all microbes, including spores. Suitable for
surgical instruments, textiles, and glassware.
- Dry
Heat Sterilization: Uses hot air at high temperatures (160–180°C) for
a longer time. Ideal for powders, oils, and instruments that may corrode
in moist conditions.
2. Chemical Sterilization
- Ethylene
Oxide (EtO) Sterilization: A gas-based method used for heat- and
moisture-sensitive medical devices. Highly effective but requires aeration
to remove toxic residues.
- Hydrogen
Peroxide Plasma Sterilization: Uses vaporized hydrogen peroxide and
plasma to sterilize medical instruments rapidly without high heat.
- Peracetic
Acid Sterilization: Involves liquid peracetic acid, commonly used for
endoscopes and surgical instruments.
- Glutaraldehyde
& Formaldehyde Sterilization: Used for high-level disinfection and
sterilization of delicate instruments.
3. Radiation Sterilization
- Gamma
Radiation: Uses high-energy gamma rays to sterilize disposable medical
supplies like syringes, gloves, and implants.
- Electron
Beam (E-Beam) Sterilization: Similar to gamma radiation but uses
high-energy electrons. Faster but less penetrating.
- Ultraviolet
(UV) Sterilization: Uses UV light to kill microorganisms. Used mainly
for surface and air sterilization rather than deep sterilization.
4. Filtration Sterilization
- Membrane
Filtration: Used for sterilizing heat-sensitive liquids and gases,
such as vaccines, antibiotics, and IV fluids.
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